Product.
Detection
BioMycoX® Mycoplasma qPCR Detection Kit
This kit is designed to detect mycoplasma contamination in research cell lines through real-time PCR.
QDR-25, QDR-50, QDR-100
Ordering Information
| Cat. No | Unit Size |
| QDR-25 | 25 Tests |
| QDR-50 | 50 Tests |
| QDR-100 | 100 Tests |
| Storage | Price |
| -20℃ | Request a quote→ |
Product Overview
Features
- Screening of mycoplasma contamination in R&D cell cultures
- Real-time quantitative PCR(qPCR) using probe(FAM labeled probe: mycoplasma, HEX labeled probe: IAC)
- Detection of 209 types of mycoplasma with high sensitivity
- Template DNA: Boiling of culture supernatant or genomic DNA extraction from cultured cells
- High Sensitivity : 10~100 copies/reaction
- Specificity : Do not detect other bacterial species with close phylogenetic relation to mycoplsma
- UDG system : Prevention of carry-over contamination
Results
Support
Certificates of analysis
Material Safety Data Sheet
Product Information
Product FAQ
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Q
What criteria should be considered when selecting a reliable mycoplasma detection kit?A
When selecting a mycoplasma detection kit, two critical factors are high sensitivity and the inclusion of an internal amplification control (IAC).
• High Sensitivity: Kits with low sensitivity may fail to detect low-level contamination, leading to false-negative results. Undetected mycoplasma can spread through cell cultures, compromising experimental outcomes and downstream applications. A high-sensitivity kit ensures that even minimal contamination is reliably detected.
• Internal Amplification Control (IAC): The IAC verifies that the PCR reaction is functioning correctly and that sample-specific inhibitors are not interfering with detection. Even if no mycoplasma is present, the presence of a clear IAC band confirms the assay worked properly, providing confidence in negative results.
By prioritizing both sensitivity and a reliable IAC, researchers can confidently maintain contamination-free cultures and ensure accurate experimental results. -
Q
Which channels should be selected on a real-time PCR instrument?A
This product uses the hydrolysis probe method and requires all two channels per well: FAM and HEX. If the instrument does not have a HEX channel, VIC may be used as a substitute.
