Product.
Detection
BioMycoX® Mycoplasma PCR Detection Kit
This kit is designed to detect mycoplasma contamination in research cell lines through conventional PCR.
D-25, D-50, D-100
Ordering Information
Product Overview
Features
- Screening of mycoplasma contamination in R&D cell cultures
- Detection of 209 types of mycoplasma with high sensitivity
- Template DNA: Boiling of culture supernatant or genomic DNA extraction from cultured cells
- High Sensitivity : 10~100 copies/reaction
- Specificity : Do not detect other bacterial species with close phylogenetic relation to mycoplsma
- UDG system : Prevention of carry-over contamination
Results
Lane 1: 100bp ladder
Lane 2: 104 copies
Lane 3: 103 copies
Lane 4: 102 copies
Lane 5: 101 copies
Lane 6: 1 copy
Lane 7: Positive Control
Lane 8: Negative Control
- When mycoplasma contamination exists, a band with around 250-300 bp appears.
- An internal DNA band with around 700 bp means the right performance of PCR reaction.
Support
Certificates of analysis
Material Safety Data Sheet
Product Information
Product FAQ
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Q
What criteria should be considered when selecting a reliable mycoplasma detection kit?A
When selecting a mycoplasma detection kit, two critical factors are high sensitivity and the inclusion of an internal amplification control (IAC).
• High Sensitivity: Kits with low sensitivity may fail to detect low-level contamination, leading to false-negative results. Undetected mycoplasma can spread through cell cultures, compromising experimental outcomes and downstream applications. A high-sensitivity kit ensures that even minimal contamination is reliably detected.
• Internal Amplification Control (IAC): The IAC verifies that the PCR reaction is functioning correctly and that sample-specific inhibitors are not interfering with detection. Even if no mycoplasma is present, the presence of a clear IAC band confirms the assay worked properly, providing confidence in negative results.
By prioritizing both sensitivity and a reliable IAC, researchers can confidently maintain contamination-free cultures and ensure accurate experimental results. -
Q
What is the concentration of the Positive Control DNA?A
The exact concentration and composition of the Positive Control DNA are not disclosed. Band intensity in PCR or Ct values in qPCR may vary depending on laboratory conditions, equipment, and reagent performance.
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Q
Why is the internal control (IAC) band missing when the mycoplasma band is visible?A
In cases of heavy mycoplasma contamination, the IAC band may not be visible due to competitive amplification. The mycoplasma band may appear stronger while the IAC band appears faint or absent. This result still indicates that the PCR reaction was successfully performed.
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Q
How should I interpret a test result where the mycoplasma band is not visible in the Positive Control?A
The Positive Control verifies proper test performance and should show both bands. If the mycoplasma band is absent, it may be due to DNA degradation caused by prolonged exposure to room temperature, vortexing, or repeated freeze–thaw cycles. For consistent results, store Positive Control DNA in aliquots and handle it gently.
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Q
How should I troubleshoot when no bands are visible in the sample result?A
The absence of all bands may indicate the presence of PCR inhibitors in the sample (e.g., high EDTA concentration, elevated FBS content, or proteins). Additional wash steps or DNA preparation using a DNA extraction kit is recommended before repeating the test.
