Product.
Detection
MycoQSearch™ Plus Mycoplasma qPCR Detection Kit
This kit is designed to detect mycoplasma contamination in production cell lines and biopharmaceuticals through real-time PCR with high sensitivity. This product can be used as an alternative to conventional culture methods especially for the qualification of ATMPs. It has Dual probe system identifying cross-contamination of positive control DNA.
QDP-100, QDPEP-100
Ordering Information
Product Overview
Features
- Mycoplasma test in production cell-lines and biopharmaceuticals (Compliance with Korea MFDS, EP and JP)
- Real-time quantitative PCR(qPCR) using probe
(FAM labeled probe: mycoplasma, HEX labeled probe: IAC, Cy5 labeled probe : Positive Control) - Verify cross-contamination: Confirm false positive reaction due to Positive Control DNA in Cy5 channel
- High Sensitivity : 1~10 copies/reaction
- Specificity : Do not detect other bacterial species with close phylogenetic relation to mycoplsma
- UDG system : Prevention of carry-over contamination
Results
Amplification Result
Tested with Quantitative M.pneumoniae Genomic DNA (Cat. No. QPGD, CellSafe)
Support
Certificates of analysis
Material Safety Data Sheet
Product FAQ
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Q
What is the difference between the Plus kit and the regular kit?A
The Plus kit includes the functionality of detecting cross-contamination caused by positive control DNA using the Cy5 channel. This feature helps identify contamination that may occur due to positive control DNA during the experiment.
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Q
Real-Time PCR instrument does not have the HEX dye. What should I do?A
Thermofisher instruments utilize a patented dye, VIC, for the HEX wavelength range. For devices that do not support the HEX wavelength, there is a HEX plate product available that can be installed to accommodate this wavelength. Please contact the head office for product purchase and use.
If you want to use the product without a separate purchase of the HEX plate, you can choose the VIC wavelength and experiment. -
Q
There are two types of ROX dye in the product, which one should I use?A
The choice of ROX dye depends on the instrument you are using. Please refer to the table below to determine the appropriate ROX dye for your specific instrument.
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ROX Type Brand Model No ROX BioRad iCycler, MyiQ, MiQ 2, iQ 5, CFX-96, CFX-384 MJ Research Opticon, Option2, Chromo4, MiniOpticon Qiagen Roto-Gene Q, Roto-Gene3000, Roto-Gene 6000 Eppendorf Mastercycler realplex Illumina Eco RealTime PCR System Roche LightCycler 480, LightCycler 2.0 High ROX ABI 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast, StepOne, StepOne plus Low ROX ABI 7500, 7500 Fast, QuantStudio(1, 3, 5, 6, 7) Stratagene MX3000, MX3005P, MX4000 -
Q
How should I select the channels on the PCR instrument?A
For this product, which operates based on the TaqMan probe method, conduct the experiment by selecting 2 channels per well (FAM, HEX) on the PCR instrument.
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Q
There seems to be a false positive result due to a PCR product from a previous experiment. Is there a solution? (Carry-over contamination solution)A
The 2X PCR master mix includes dUTP instead of dTTP, so the UDG system is applied, and it can be solved with the following contents.
1) Treatment of UNG (Uracil-N-glycosylase, not included in kit) in PCR products (amplicon) containing dUTP
2) UNG hydrolyzes uracil-glycosidic bonds in the DNA regions containing dUTP, preventing re-amplification of PCR products.
3) The UDG system ensures that the main DNA containing dTTP remains intact, preventing carry-over contamination (UDG=UNG).
